Research Assistant, Senior bei Vanderbilt University
Vanderbilt University · Nashville, Vereinigte Staaten Von Amerika · Onsite
- Professional
- Optionales Büro in Nashville
The Research Assistant, Senior is part of the Lau Lab in the Department of Cell and Developmental Biology at Vanderbilt University. This position provides key technical support for the lab’s experimental research in gastrointestinal biology and tissue modeling. The primary role is to contribute to both in vivo and in vitro workflows while supporting broader lab goals and priorities. This includes performing molecular biology techniques, staining, microscopy, organoid culture and in vivo mouse experiments and management.
Day-to-day responsibilities include molecular biology (e.g., PCR), mouse colony management, tissue dissection, and supporting hybridization chain reaction fluorescence in situ hybridization (HCR-FISH). The Research Assistant, Senior plays a crucial role in maintaining lab operations, working closely with multiple lab members, alternating between projects as needed based on priorities and personnel availability.
For experimental tasks, the Research Assistant, Senior will support molecular biology research focused on mouse experiments and relevant data collection. They will also be responsible for mouse colony management and other molecular assays, specifically staining and microscopy.
Duties and Responsibilities
Molecular biology – DNA extraction and PCR-based Genotyping
Single cell sequencing
Aseptic survival surgeries on small animals
Gastrointestinal organoid cell culture
Oral gavage, Injectable treatments on small animals for experimental purposes
Mouse colony management
Mouse handling, genotyping, treatments, ear tagging, tailing
Maintaining mouse colony records
HCR-FISH & IF – antibody staining
Intravital microscopy
Cut and process tissue slides for histological and imaging studies
Mouse dissection and tissue collection
Cryopreservation of experimental tissue, different cell lines and organoids
Cryosectioning
Working with Human patient-derived material to create single cells for DNA sequencing, Organoids and other cell culture work
Day-to-day lab management such as keeping instruments running and ordering supplies § routine lab work such as buffer stock preparation, aliquoting reagents, maintaining electronic records, molecular biology
Analyzing Research data and Updating research data
Supervisory Relationships:
This position does not have supervisory responsibility, this position reports administratively and functionally to the PI, Ken Lau.
Qualifications
- Bachelors in a biology field is strongly preferred.
- Molecular biology skill is strongly preferred.
- Mouse experience is strongly preferred.
- 4 years of lab experience is strongly preferred
Company
At Vanderbilt University , our work - regardless of title or role - is in service to an important and noble mission in which every member of our community serves in advancing knowledge and transforming lives on a daily basis. Located in Nashville, Tennessee, on a 330+ acre campus and arboretum dating back to 1873, Vanderbilt is proud to have been named as one of “America’s Best Large Employers” as well as a top employer in Tennessee and the Nashville metropolitan area by Forbes for several years running. We welcome those who are interested in learning and growing professionally with an employer that strives to create, foster and sustain opportunities as an employer of choice.
We understand you have a choice when choosing where to work and pursue a career. We understand you are unique and have a story. We want to hear it. We encourage you to apply today so that you might become a part of our story.
