Cell Line Development Analyst chez Mabxience

Responsible for the proper execution of activities related to the development of cell lines for the generation of stable, monoclonal and high-expression cell lines that produce recombinant monoclonal antibodies, fusion proteins, hormones, biosimilar molecules of non-antibody proteins, etc.

Provide technical leadership for analysts involved in cell line development. Contribute to extensive interaction with cross-functional teams; Upstream process development, downstream process development, and analytical development.

1. Responsible for organizing the daily laboratory activities, providing support for the proper execution of the different projects.
2. Execution of cell line development activities such as:
   • Aseptic management of mammalian cell lines, including routine thawing, maintenance, and subculture of mammalian cells.
   • Management of mammalian cell cultures in different stages: static and agitated.
   • Preparation of culture media, feed, and cell culture solutions.
   • Creation of cell phone banks.
   • Generation of stable and transient transfections.
   • Cell clone isolation and high-productivity clone selection.
   • Monoclonality data generation with the help of a cellular imager.
   • Execution of the production process (fed-batch) in stirring flask and/or micro bioreactor system.
   • Cell line stability studies
   • Media panel and feed screening for title enhancement in clones.
   • Data collection, analysis, and creation of appropriate statistical presentations, including graphs and tables.
   • Maintain the laboratory in correct conditions following GLP standards.
   • Preparation, maintenance and drafting of Operating Procedures of Standards, preparation of Cell Line Development report, etc.
   • Continuous improvement: You will support improvements throughout the cell line development process.

1. Education: Degree in the relevant major of Biology/Biotechnology
2. Languages: Fluent Spanish, working knowledge in English (mandatory English level B2, part of the interview will be conducted in English), knowledge of other languages will be an asset.
3. Experience (years/area): +1 year of relevant experience in mammalian cell line culture activities. Experience in cell line development and/or having been working with pharmaceutical biologics and biotech environments is a plus.
4. Specific knowledge:

• Experience in cell line development activities, such as cell line subculture and maintenance, cell banks, production process (fed-batch), transfection, generation of cell pools with stable transfection, cell line stability studies. Knowledge of molecular biology techniques is a plus.
• The work with the isolation and analysis of data from cell clones will be positively valued.
• Knowledge of regulatory guidelines for cell line development.
• Knowledge about mammalian cell line metabolism, as well as the structure, function of monoclonal antibodies. Specific understanding of post-translational modifications.

1. Travel: Not mandatory.
2. Personal Skills:

• Ability to learn quickly in terms of laboratory techniques for cell line development.
• Ability to read and carefully follow instructions in English.
• Communication skills with laboratory staff, superiors, and with staff from other laboratories.
• Excellent problem-solving skills and organizational skills.
• Availability for work on weekends and holidays (subject to process requirements).
• Availability to work in morning and afternoon shifts (under process requirement).